Chromosome aneuploidy in the spermatozoa of two men with globozoospermia.

نویسندگان

  • F Morel
  • N Douet-Guilbert
  • A Moerman
  • B Duban
  • C Marchetti
  • B Delobel
  • M-J Le Bris
  • V Amice
  • M De Braekeleer
چکیده

The objective of this study was to determine the aneuploidy level in spermatozoa in two men with globozoospermia. Sperm nuclei were analysed by fluorescence in-situ hybridization (FISH) in two infertile males with globozoospermia. Dual FISH for chromosomes 7 and 9, 13 and 21, and triple FISH for chromosomes X, Y, and 18 was performed. The main outcome measured was meiotic segregation differences between both globozoospermic men and controls. A statistically significant difference in disomies 13 and 21 was found between patients 1 and 2. The diploidy rate of spermatozoa of patient 1 (0.876%) was significantly increased compared with that of patient 2 (0.304%) and control men (0.293%). In conclusion there seems to be a slightly increased frequency of aneuploidy in round-headed spermatozoa. However, it is unlikely that these aneuploid spermatozoa would be used in assisted reproduction techniques.

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عنوان ژورنال:
  • Molecular human reproduction

دوره 10 11  شماره 

صفحات  -

تاریخ انتشار 2004